项目名称：基于手性响应性聚合物的新型色谱固定相的开发及其在糖手性分离中的应用
项目来源：国家自然科学基金面上项目
项目编号：21275114
项目金额：80万元
糖类物质种类繁多、结构复杂、而且具有高度的手性，在糖化学研究中，一个首要迫切的课题就是复杂糖组分的分离与纯化。各种D型单糖、寡糖的生理作用已被广泛揭示，然而对应的L 型糖的研究却十分匮乏，缺乏有效的手性糖对映体分离手段是制约L 型糖研究的重要因素，对于材料学家和化学家还是一个挑战。在申请者前期手性响应性聚合物材料研究基础上，本项目将开发一种基于短肽体系的能对糖分子的手性产生响应的手性聚合物材料，并将其开发为手性色谱固定相，试图通过材料对目标糖分子立体选择性的作用，以及材料浸润性的大幅转变，实现对单糖手性对映体的分离。并在此基础上，进一步实现对多种手性二糖、寡糖、多糖、简单糖肽的分离和纯化。这种新型手性色谱柱，同时具有手性选择性好、载料量大、可通过环境条件的改变对分离过程进行智能控制、易洗脱、可逆性好等优点，为解决糖类物质的分离提供了一种全新的策略，也是手性响应性材料在分离领域中的一次很好的应用。
Title of funded project：Development of chirality-responsive polymer based chromatographic stationary phases and their applications in enantiomeric separations
Funding agency：National Natural Science Foundation of China
Grant number：21275114
Total funding amount：CNY 800,000
Plentiful saccharides and their derivatives usually have complex structures, and highly specialized chirality. In the research of saccharide chemistry, one of the urgent topics is the separation and purification of saccharide components. On the other hand, the biological and physiological properties of various D-monosaccharides and oligosaccharides have been explored systematically, however, their respective L-enantiomers have not received sufficient focus although their potential roles in the physiological pathway still deserve in-depth investigation. How to enantioselectively recognize and separate these saccharide enantiomers is the critical factor limiting the research of L-saccharides, which is still a big challenge for materials scientists and chemists. On the basis of our previous works in the chirality-responsive polymeric materials, we plan to develop a series of chiral polymers in this project, the key components of which are the chiral peptide units with the capabilities of responding to the targeted saccharide enantiomers.
Then these polymers will be grafted on silica oxides microspheres and developed to be the chromatographic chiral stationary phases, which are designed to separate the enantiomers of saccharides utilizing the highly stereoselective interactions between materials and targeted species, as well as the obvious transformation of wettability of materials. In the next stage of this project, the separation performance of these novel chiral stationary phases towards various chiral disaccharides, oligosaccharides, polysaccharides and some simple glycopeptides will also be evaluated systematically. Compared with the traditional materials, this kind of chrial stationary phase will have obvious advantages, such as excellent enantioselectively, high loading efficiency, satisfied reversibility, rapid elution, while the dynamic separation process could be controlled conveniently and intelligently according the stimuli in the surrounding environment. Due to these reasons, it is believed that introducing chirality responsive materials into the field of separation will be an excellent application for this kind of material, which also provides an original strategy for the separation of various saccharides and their derivatives.
